Hong-Qing Ling, Hui Liu, Biao Ma, Hua Sun, Zhensheng Li, Aimin Zhang, Daowen Wang,
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
Bread wheat (Triticum aestivum), one of the most widely cultivated and consumed food crops in the world, is a hexaploid containing A, B and D genomes. Due to its complex polyploidy nature and large genome size (17 Gb), the genetic and functional analysis of bread wheat is extremely challenging. Triticum urartu is the progenitor of wheat A genome. It plays a central role in wheat evolution, domestication and genetic improvement because the A genome is the basic genome of bread wheat and other polyploidy wheats. We sequenced T. urartu accession G1812 using a whole-genome shotgun strategy on the Illumina HiSequation (2000) platform, and generated a draft genome of wheat A genome (Ling et al., 2013, Nature 496: 87-90). To completely sequence the wheat A genome, we constructed 3 BAC libraries and created BAC contigs using the whole genome profiling technology. Based on BAC contig data, we selected 47,223 BACs by MTP approach, and sequenced them. Additionally, we also generated >80 G sequence data by whole genome sequencing using PacBio RS II. The sequence assembly has been finished and anchored on their corresponding chromosome with a high resolution SNP map. In conclusion, completing the genome sequence of T. urartu will provide a diploid reference for the analysis of polyploidy wheat genomes, and is a valuable resource for the genetic improvement of wheat to meet the future challenges of global food security and sustainable agriculture.