Samy A. Selim
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka, P.O. 2014, Saudi Arabia
Plesiomonas has been implicated as an agent of human gastroenteritis for many years, with an increasing number of reports describing such infections during the recent years. The present project proposal study the REP-PCR-DNA fingerprints profiling genes in Plesiomonas species isolates collected from clinical samples in Saudi Arabia.
Specimens included blood, pus, tissues and body fluids, urine, sputum and peritoneal dialysate. Antimicrobial susceptibility was determined by the Kirby- Bauer disk diffusion method. Identified at least to genus level by MicroScan® microbiology, biochemical tests, API 20E system (bioMe´rieux, France). Nucleic acids isolated by lysis method. All the isolates were subjected to REP-PCR typing method for finding common REP-types among all of the isolates. Antimicrobial susceptibility tests showed that Plesiomonas presents a natural resistance to β-lactams. Furthermore, some strains may carry genes for resistance to tetracyclines. REP-PCR-DNA fingerprints of all the isolates revealed the presence of four Plesiomonas sp. lineages; 82% of all the isolates belonged to three dominant lineages. Genotype (A) isolates predominant and showed increased antibiotic tolerance to amoxicillin and ciprofloxacin compared with the other isolates. These studies indicated that there is considerable flux in the antibiotic resistance genes found in the beta lactam drugs in Plesiomonas sp.
Keywords: Antibiotic Resistance, Gene Clusters, Plesiomonas.