Jojula Malathi and Shravan Kumar Gunda
Sri Shivani College Of Pharmacy, Warangal, India
The Mycobacterium tuberculosis contains Mycolic acids which represents a major component of the unique cell wall of mycobacteria. Isoniazid is frontline drugs used in the treatment of tuberculosis (TB) and play a vital role in inhabiting mycolic acid biosynthesis, INH is inactivated by mycobacterial and human arylamine N-acetyltransferase (NAT). The study of protein structure information provides fundamental aspects into most biochemical functions and consequently into the cause of diseases and possible treatment. Most of the protein structures were not solved experimentally, for this it requires X-ray crystallography, NMR and electron microscopy. Hence, in silico protein structure prediction methods are step into generate a protein structure. Molecular modeling of proteins is a rapidly growing. The present study deals with homology modeling of isolated NAT protein of mycobacterium by using Modeller 9.15. The model shows that 92.5% of amino acids in most favored region. NAT is critical for mycolic acid synthesis and hence other derivative cell wall components and represents a novel target for antituberculosis therapy.
Keywords: NAT, INH, Mycolic acids, biomarkers.