Department of Chemistry, Faculty of Art and Science, Gaziosmanpasa University, 60240 Tokat, Turkey
Origanum, belonging to the Lamiaceae family consists of 23 species and 6 hybrids in the Turkey flora, 14 of which are endemic1. Both plants materials were collected from Antalya, Turkey. Aerial part of the O. bilgeri was dried and powdered then extracted with hexane, ethyl acetate, methanol and water individually. The aqueous part of water extract was extracted with ethyl acetate to yield the water to ethyl acetate extract. Rosmarinic acid (1), apigenin-7-O-glucoside (2), eriodictyol (3), Globoidnan A (4), 5,6,3'-trihidroksi-7,8,4'-trimetoksiflavon (5) were isolated from water to EtOAc by chromatographic methods including column chromatography and HPLC. The spectroscopic methods (1D, 2D NMR, LC-TOF/MS) were applied to identify the isolated compounds. Aerial part of O. solymicum was boiled in distilled water, filtered then aqueous part partitioned with ethyl acetate and n-butanol sequentially. 3,4-dihdroxy benzoic acid (6), 3,4-dihydroxy benzaldehyde (7), caffeic acid (8), rosmarinic acid (1), lithospermic acid (9) were isolated from ethyl acetate extracts. The antioxidant effects of isolated compounds were studied using DPPH•, ABTS•+, reducing power, metal chelating assays. All isolated compounds exhibited from moderate to excellent antioxidant activities.