Mohammad Javad Najafzadeh, Anna Kolecka, Majid Alizadeh, Hossin Zarrinfar and Abdolmajid Fata
Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Background: vaginal candidiasis (VVC) is a common problem in women. The purpose of this study was to identification of Candida species isolated from vaginitis women referred to Ghaem Hospital by MALDI TOF mass spectrometry techniques.
Methods: The experiment was performed on 65 Candida speceis isolated from women in Ghaem Hospital. First, all specimens identified using phenotyping techniques such as microscopy, germ tube test and culture on Chrom agar and corn meal agar medium, then Candida species were rapidly identified and evaluated with MALDI TOF mass spectrometry.
Results: Of the 65 isolates analyzed, 93.8% were correctly identified by MALDI-TOF mass spectrometry. In this study, the most frequently isolated species were Candida albicans (58.5%), followed by Candida tropicalis (16.9%), Candida glabrata (7.7%), Candida parapsilosis (7.7%), Candida guillermondii (3.1%) and species not in database (6.1%).
Conclusion: Our results demonstrate that the MALDI TOF mass spectrometry is a fast and reliable technique, and has the potential to replace conventional phenotypic identification of Candida species and yeast strains routinely isolated in