Amr K. Elfayomy, Shaima M. Almasry, Shereen A. El Tarhouny and Magda A. Eldomiaty
Department of Obstetrics and Gynecology, Taibah University, Al-Madinah Al-Munawarah, Saudi Arabia
This study aimed to isolate mesenchymal stem cells (MSC) from human umbilical cord blood (HCB) and to explore their influence on ovarian surface epithelium and milieu after paclitaxel induced ovarian failure. The HCB cells were cultured and expanded in vitro, and studied for CD34, CD44, and Oct ¾ using flow cytometry. PCR analysis revealed positive human gene; beta-actin expression in all rats’ ovaries injected with HCB-MSC. After 4 weeks of transplantation, histological analysis revealed normal organized surface epithelial and tunica albuginea cells and increased antral follicle count. There was a significant reduction in FSH, elevation in E2 serum levels. Immunohistochemical analysis revealed a significant increase in integrated optical densities (ID) of cytokeratin (CK) 8/18, transforming growth factor (TGF)-ß, and proliferating cell nuclear antigen (PCNA) expression, decrease in ID of caspase (CASP)-3 expression compared to the paclitaxel groups. We concluded that HCB-MSC can restore the ovarian hormonal levels and follicular development after paclitaxel injection through a direct triggering effect on the ovarian epithelium and/or indirect improvement of the ovarian niche through the enhanced expression of CK 8/18, TGF-ß and PCNA. These molecules are crucial in regulating the formation of primordial follicles and suppressing CASP-3 expression and follicular cell apoptosis.
This study was supported by Grant No. 173/34 from King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia.