Takfarinas Idres, Karim Benhenia, Nassima Bedrani, Fella Amari and Fatma Zohra Yakoub
Short term conservation of sheep semen is a process that will extend the life of sperm, however this life remains limited, which reduces the development of artificial insemination in sheep, only a suitable storage media manage to overcome this constraint. The objective of this experiment was to test refrigeration at 4 ° C in epididymal semen ram, relying on three storage media: based egg yolk, 5% glycerol and 15% glycerol. Our method of collection was given to the retrograde flushing of the epididymis, the semen collected is evaluated and then diluted and tested in 04 conservation community, a witness, consisting of media (Tris Acid Fructose citrique), the second comprises the basal media and 20% egg yolk and the last two are the basal media, 20% of egg yolk and a glycerol concentration of 5% and 15% respectively. Later we mixed 1 volume of diluted semen with 9 volumes of each storage media. Finally, we evaluated the individual motility and membrane integrity (HOST) of the four environments: 0 h, 6 h, 18 h, 24 h, 36 h, 48 h, 56 h and 72 h. Our results reveal that the maximum period the seed of conservation without the addition of cryoprotectants is 18h (HOST: 65.66%), however the media containing only non-penetrating cryoprotectant (yolk) saves acceptable quality semen for 36 h (HOST: 58.12%). The joint use of a cyoprotecteur penetrating and non-penetrating, the concentration of the latter 5% allows the protection of the quality of epididymal semen until 36h (HOST: 58.12%), while only 15% glycerol significant toxicity occurs from 18h refrigeration (HOST: 40%). We conclude that the addition of a penetrating and non-penetrating cryoprotectant optimizes seed shelf life and that the concentration of 5% glycerol appears to be the proper fit.
Sheep semen, sperm, refrigeration, glycerol.