Esrafil Mansouri, Mohammad-Ali Assarehzadegan, Fereshteh Nejad-Dehbashi, Wesam Kooti
Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical
Sciences, Ahvaz, Iran
The aim of this study, was to evaluate the effects of pravastatin on Adriamycin (ADR)-induced nephropathy and the mechanisms involved. Forty rats were divided to 4 groups: control, ADR (15 mg/kgbw, intraperitoneal injection), ADR plus pravastatin (20mg/kgbw that started 5 days before ADR injection), ADR plus pravastatin (20mg/kgbw that started 5 days after ADR injection). On day 20 after ADR injection animals were sacrificed and biochemical parameters of urine and blood were measured. Renal morphology was examined by light microscopy and also, Real-time PCR and Western blotting were used to measure expression of glomerular nephrin mRNA and protein respectively. The results showed that administration of pravastatin in both groups, decreased (p < 0.005) levels of 24-h urinary protein (24-h UP), blood urea nitrogen (BUN) and creatinine which were increased following the injection of ADR, also pravastatin reversed structural changes seen in ADR group. Furthermore, pravastatin elevated expression of glomerular nephrin mRNA (p < 0.01) and protein which had reduced in ADR group. We conclude that pravastatin can protect the kidneys from damages caused by ADR.
Keywords: Nephropathy, adriamycin, pravastatin.