Ali Rezaei-Matehkolaei, Ali Zarei Mahmoudabadi, Koichi Makimura, Yvonne Graser and Abbas Jahangiri
Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical
Sciences, Ahvaz, Iran
The dermatophytes are a group of keratinophilic molds; some of which cause infections known as dermatophytosis in man and animals. The data regarding the distribution profile of soil microbiota in Iran are rare. The aim of this survey was to broaden the knowledge about distribution of geophilic dermatophytes in soils of an area of Khuzestan province, Southwestern Iran. The samples were collected from 160 different spots with likely presence of soil with humans and animals keratin residues and the PH of each sample was measured. Isolation of keratinophilic dermatophytes was accomplished based on the hair-baiting technique and the isolates were identified according to the macro- and micro-morphological properties of the colonies on Mycobiotic agar medium. All isolates, morphologically screened as dermatophyte, were subjected to PCR-sequencing of the nuclear rDNA ITS regions. Totally, in 36 (22.5%) samples a dermatophytes strain was isolated. Surprisingly, sequencing revealed that the geophilic species Microsporum fulvum was the most dominant isolate (n=32; 88.8%) and other species included M. gypseum, and the zoophilic species M. canis, Trichophyton interdigitale and Arthroderma benhamiae (each one 2.8%). In view of PH, most of the isolated strains were from weakly acidic to neutral or alkaline soils. The predominance of M. fulvum as dermatophytes species isolate from soil has not yet been reported worldwide.
Keywords: Dermatophyte, soil, Microsporum fulvum.