Mesut Karahan, Pelin Pelit Arayıcı, Tayfun Acar, Zeynep Akdeste Mustafaeva, Martina Frimmelova and Rudolf Toman
Department of Bioengineering, Faculty of Engineering and Natural Sciences, Uskudar University,
Complexation and conjugation of biopolymers with lipopolysaccharides, proteins, peptides and other biomolecules introduce several innovations for applications in the fields of medicine, pharmacy, biotechnology and bioengineering [1-3]. Examination of polymer-biomolecule conjugates and complexes is very important for understanding the behavior of conjugates and complexes in the living organisms, and the development of bioconjugation technology. Coxiella burnetii is etiological agent of Q fever. The bacterium causes a most frequent diseases of rickettsial origin in the world. The most common acute form of Q fever is characterized as a sudden onset of fever, chills, fatigue, headache, loss of appetite or less frequently as a granulomatous hepatitis, with a significant incidence of neurologic complications. Persistent infections in humans may lead to chronic form of Q fever, which may be associated with endocarditis. C.burnetii-induced endocarditis is often fatal. C.burnetii contains a lipopolysaccharide (LPS) which is considered to be a major factor of virulence of the bacterium. For the purpose of this study, the LPS was isolated from the C.burnetii strain Nine Mile I (RSA 493), serologically in virulent phase I [4-7]. A series of experiments have been performed to develop a polymer-LPS complex, which should form a basis for the construction of synthetic vaccine against Q fever.
Our preliminary results have shown that it is now possible to prepare an LPS- polyoxidonium complex as a possible vaccine prototype. In future, it is planned to examine efficiency of this candidate vaccine in vitro and in vivo studies.
Keywords: Biopolymers, coxiella burnetii, ternary copper metal complexes, vaccine
This 2513-TUBITAK and Slovak Academy of Sciences Bilateral Cooperation Project is supported by TUBITAK under the name of ''Development of Lipopolysaccharide-Biopolymer Complex and Conjugates Against the Q Fever Disease For Application Purpose of Vaccine Prototype and Diagnostic Kit'' with the project number of 113Z938.
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