Sayed Sartaj Sohrab, Parthasarathi Bhattacharya and Debashis Ranab
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
Wild tomato species (Solanum peruvianum) are known to harbor tospovirus resistant genes. An attempt was made to Introgress resistant genes into cultivated tomato (Solanum lycopersicum) lines but due to incompatibility, fertilization barriers and embryo abortions the successful introgression and development of interspecific hybrid are hampered. To overcome this barrier; embryo rescue technology was adopted in this study. The crossed fruits were harvested from cultivated tomato line at different time intervals (7-35 days) and immature embryos were harvested from sterilized fruits under aseptic condition and cultured on standardized culture medium for 25 days containing MS + BAP (2.0mg/l),Yeast extract (1gm/l), Sucrose (3%) solidified with 0.3% gelrite (0.3%). The immature embryos formed callus and small shoots were regenerated from callus on same medium within 24-25 days. Further the emerged shoots were sub-cultured on shoot multiplication medium for 10-15 days supplemented with MS + BAP (2mg/l)+IBA (0.1mg/l).The small shoots were separated and further elongated on shoot elongation medium containing MS +BAP (0.3mg/l). The well-developed and elongated shoots developed profuse roots after 7-14 days cultured on rooting medium containing MS+NAA (0.1mg/l) and interspecific hybrid tomato plants were fully developed and hardened into MS liquid medium without any hormone and finally shifted to small cups containing sterilized vermiculite soil mixture and incubated at room temperature for complete acclimatization. The best regeneration capability was observed in 31 days old immature embryos. The interspecific nature of developed tomato plant was further confirmed by their morphologic characters as compared to their parents.