Su Jeong Baek, Dong-Gi Lee and Jong-Soon Choi
Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, South Korea
Aging is a multifactorial process resulting from the accumulation of cellular damage over time, leading to physiological deterioration, increased mortality and eventual death. Though there are many theories on aging, the free radical theory about cellular senescence was focused on the present study. Ginseng among the many health foods is well known in herbal medicine as a tonic and restorative agent. Especially red ginseng is very popular over thousands of years as a healthy food. So we discuss that what is the connection red ginseng and antiaging. Ginsenoside Rg3(S) in red ginseng decreased native and hydrogen peroxide (H2O2)-induced reactive oxygen species (ROS) in both young and old human dermal fibroblasts (HDFs). Interestingly, the pre-treatment of Rg3(S) did not affect the down-regulation of H2O2-induced ROS production whereas the post-treatment of Rg3(S) dramatically decreased the intracellular ROS levels. To identify the Rg3(S)-altered proteins in HDFs, the label-free quantitative proteomics was applied to obtain the time-dependent (2, 6, and 12 hours) proteome profiles after the treatment of Rg3(S) to old HDFs. Nano-UPLC-high definition mass spectrometry revealed 129 significant proteins upon 6 hour treatment of Rg3(S) which were classified into the molecular functions with antioxidant activity, binding and catalytic activity. Among the identified proteins, the core proteins with radical scavenging activity included peroxiredoxin 1, 2, 3, 4, and 6 by proteome network analysis. Most of aging-related free radical scavenging proteins exhibited similar expression patterns in terms of their mRNA and protein levels. Therefore, the ginsenoside Rg3(S) is suggested to play a direct scavenger of intracellular ROS in HDFs and would be able to therapeutic agent against oxidative stress.
Keywords: Aging, ginsenoside Rg3, human dermal fibroblast, proteomics.