Ramazan Erenler and Ozkan Sen
Department of Chemistry, Faculty of Art and Science, Gaziosmanpasa University, Tokat, Turkey
Cynanchum genus (Asclepiadaceae family) consists of almost 200 species distributing in Africa, Mediterranean region, as well as the tropical areas of Europe and Asia . Cynanchum Acutum L. Subsp. Sibiricum (wild) Kech. F was collected from East part of Turkey. Dried and powdered aerial part of plant material (1.0 kg) was boiled with distillated water for 2h then extracted with ethyl acetate, filtered and solvent was removed by rotary evaporator to yield the extract. The extract was subjected to column chromatography (2.5 × 100) using the silica gel 60 (0.063-0.20 mm) as an absorbent. The column was eluted with solvent systems of increasing polarity from hexane to hexane-EtOAc and EtOAc-MeOH. After the repeated column chromatography and HPLC, kaempferol-3-O-α-rhamnopyranoside (1), quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3) and quercetin-3-O-β -glucoside (4) were isolated. The structures were elucidated by spectroscopic techniques, basically 1D-NMR, 2D-NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds and water-soluble ethyl acetate extract were determined by xCELLigence using the HeLa ((human uterus carcinoma) cell lines. This study presented the antiproliferative activity of isolated compounds on HeLa cell lines.
This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, 113Z853).
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