Uma Devi M., Malathi J., Shravan Kumar G., B. Bhima and Sireesha T.
Department of Microbiology, Osmania University, Hyderabad, India
Mycobacterium tuberculosis is remarkably skillful in surviving inside host macrophages and altering the macrophagial gene expression for its own benefit. fadD30 is cell wall molecule which encodes Long chain fatty acid AMP ligase. Fatty acids are known to be responsible for the mycobacterial growth, survival, and pathogenicity. To know the activity of such novel genes by experimental studies is time taking such as X-ray crystallography, NMR and electron microscopy. Hence, insilico protein structure prediction methods are step into generate a protein structure. Molecular modeling of proteins is a rapidly growing. With this back ground the present study deals with homology modeling of isolated fadD30 protein of Mycobacterium tuberculosis by using Modeler 9.15. The model shows that 86.8% of amino acids in most favored region. fadD30 is critical for fatty acid biosynthesis which represents a novel target for antituberculosis therapy.
Keywords: MTB, cell wall protein, anti tuberculosis, fadD30.