Mesut Sohrab,Pelin Pelit Arayıcı and Akdeste Mustafaeva
Department of Bioengineering, Faculty of Engineering and Natural Sciences, Uskudar University, Istanbul, Turkey
Conjugation of weak immunogenic antigens to different origin biopolymers, development of novel and efficient polymer systems, preparation and application of specific controlled dispersion formulations bring several innovations in medicine, biotechnology and bioengineering fields. Recently, water-soluble complexes and conjugates are used intensively on many issues such as increasing enzyme stability, drug delivery systems, polymeric vaccine systems, diagnostic kits, etc [1-4].
We aimed to develop a potential vaccine candidate against rabies by covalently conjugating Polyacrilic acid (PAA) with an antigenic peptide epitopes of rabies virus protein. Therefore in this study rabies virus protein’s peptid epitope 31D (WAVYTRIMMNGGRLKRC) sequence which shows antigenic properties was synthesized with solid phase peptide synthesis methods using microwave energy in the peptide synthesizer (Liberty Microwave Assisted Solid Phase Peptide Synthesis Tool). Purified and characterized peptide’s water-soluble PAA-peptide bioconjugates were synthesized in the presence of crosslinker “1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride” (EDC) with PAA. In this study, poly (N-vinyl-2-pyrrolidone-co-acrylic acid) copolymers which will be used as carrier molecule was synthesized and it’s structure-function analysis were performed. Bioconjugates were synthesized as PAA is constant, peptide has different rates (npeptide/nPAA), and peptide is constant, polymer has different rates (nPAA/npeptide), respectively. Successful conjugation was evidenced by HPLC, GPC (UV, refractive index, light scattering detectors), Zetasizer and FTIR.
The results from this study are expected to be helped for formation of straight chain peptide-polymer vaccine prototypes.
Keywords: Rabies, Synthetic peptide synthesis, Conjugation, Biopolymer, Polymer carriers.
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 Basak Iscani Eroglu, Yasemin Budama Kilinc, Zeynep Mustafaeva, Turkish Journal of Biochemistry, 2011, 36(3), 222-229.
 Karahan, M.; Mustafaeva, Z.; Ozeroglu, C., Polish Journal of Chemical Technology, 2014, 16(3), 97-105.