Marwa Gado Mohamed Badawy, Ahmed Osman and Maha Abdel-hamid
Department of Biotechnology, Cairo University, Cairo, Egypt
Acute myeloid leukemia; AML, is mostly found in adult. The survival rate in AML patients is relatively low and this remains a challenge. Thus, it is urgently needed to search for new therapies. Over the last decade, microRNAs (miRNAs) have received wide attention as a novel molecular markers, which can be used in monitoring any change in cellular microenvironment and thus have high diagnostic and prognostic potential for many diseases. MicroRNAs are a very small RNAs (18- 25 nucleotide) and they are naturally occurring in cells. They regulate gene expression by targeting mRNAs based on the degree of complementarity with their 3'UTR. In This study, the expression pattern of miRNA-29a-3p, miRNA-92a-3p, along with their putative target genes were analyzed to assess their role in the onset and/or progression of AML in adult patients. The current study included 40 adult AML patients and 10 healthy individuals, with matched ages and sexes, who served as control group of the study. Blood samples were withdrawn from all individuals. Cellular RNA was extracted and plasma samples were separated and used as a source of circulating RNA. QPCR was used for detecting the level of gene expression in blood samples. The data presented in this study revealed a negative correlation between miRNA-92a-3p, miRNA 29a-3p and their target gene (MCL1). It could be concluded that these data supported the role for miRNA-29a-3p and miRNA-92a-3p as tumor suppressor in AML, and proved the therapeutic promise of regulating miRNA-29a /miRNA-92a expression for AML in the future.
Keywords:AML, miRNAs, QPCR.