Sanghyeob Lee, Nyam-Osor Batkhuu and Ming-Gen, Wu
Bioresource Engineering, Sejong University, Seoul, South Korea
Although Camelina sativa is known to its nutritional value and the feedstock of biodiesel, genetic or genomics study is still at the beginning. The addition of a complete genome will facilitate the research of this understudied oilseed crop. In this study, we analyzed 20 accessions of Camelina spp. with genotyping-by-sequence (GBS) technology. A total of 35,783 single nucleotide polymorphisms (SNPs) were generated with strict screening steps and basic genetic studies were performed to see their diversity. STRUCTURE and phylogenetic analysis generated five subgroups with the subgroup V thought to be the most ancient. Winter-types (subgroup I) may have been diverged from summer-types. Some genomics regions were found to be negatively selected and most of them were gene-rich regions. Marker-trait associations (MTA) with plant height, leaf length, and pod size inferred a total of 154 SNPs and 72 nearby genes which are significantly associated with these phenotypes. In addition, genetic engineering approaches are being tried to improve drought resistance and fatty acid composition for mass cultivation of wasteland of Korea, China, and Mongolia.
This work was supported by a grant from the "Bio-industry Technology Development Program (111057-5, 312033-5)" of iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry).
Keywords: Camelina, Wasteland utilization, Genotyping, Drought-resistance.